Stored Renin Isoforms in the Developing Rat Kidney
نویسندگان
چکیده
منابع مشابه
Renal nerves modulate renin gene expression in the developing rat kidney with ureteral obstruction.
Chronic unilateral ureteral obstruction (UUO) in newborn rats activates renin gene expression in the obstructed kidney, and increases renin distribution along afferent glomerular arterioles in both kidneys. To investigate the role of the renal nerves in this response, 2-d-old Sprague-Dawley rats were subjected to UUO or sham operation. Chemical sympathectomy was performed by injection of guanet...
متن کاملIsolation of renin-rich rat kidney cells.
Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show ...
متن کاملFurther studies on the determination of renin in rat kidney.
THE determinations of reni are classified into wo categories : the direct and indirect method. The former is based o the elevation of bloo pressure induced by the intravenous injection of test materials directly into the animal. The pressor activity is due to angiotensin formed in vivo. In the latter, the amount of renin is determined indirectly from the assay of angiotensin produced in vitro a...
متن کاملThe storage form of renin in renin granules from rat kidney cortex.
Renin granules were partially purified from rat kidney cortex, and a storage form of renin in the granules was examined. Renin granules were isolated by discontinuous Percoll density gradient centrifugation followed by continuous Percoll density gradient centrifugation. The partially purified fraction was free from mitochondria and microsomes, as judged by the absence of marker enzymes of these...
متن کاملRenin substrate in granules from rat kidney cortex.
1. Subcellular fractions of rat kidney cortex generated angiotensin I continuously over 2h when incubated at 37degreesC with rat renin, indicating the presence of renin substrate within cells in the renal cortex. 2. Renin substrate was located in highest specific concentration in particulate fractions. The particles containing renin substrate had a sedimentation velocity slightly lower than mit...
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ژورنال
عنوان ژورنال: American Journal of Hypertension
سال: 1998
ISSN: 0895-7061
DOI: 10.1016/s0895-7061(97)00417-2